Performs multiple sequence alignments
Tutorial and training materials by OpenHelix
|Learn to use the ClustalW2 resource at the EBI Toolbox site for performing multiple sequence alignments, or MSA. MSA allow us to analyze the evolutionary relationship between species on a molecular sequence level. As well, multiple sequence alignments can help us to identify functionally important positions in a sequence family by simply looking at the conservation of positions or motifs within the sequence data.|
Download the example data file by clicking here.
- some background and theory for multiple sequence alignments
- how to conduct sequence alignments using
- to understand and adjust the results of ClustalW2 alignments
Recent BioMed Central research articles citing this resource
Niemi Marianna et al., Mitochondrial DNA and Y-chromosomal diversity in ancient populations of domestic sheep ( Ovis aries ) in Finland: comparison with contemporary sheep breeds. Genetics Selection Evolution (2013) doi:10.1186/1297-9686-45-2
Godshall G Brian et al., An aggregate analysis of many predicted structures to reduce errors in protein structure comparison caused by conformational flexibility Selected articles from the Computational Structural Bioinformatics Workshop 2012 Computational Structural Bioinformatics Workshop 2012. BMC Structural Biology (2013) doi:10.1186/1472-6807-13-S1-S10
Kettani-Halabi Mohamed et al., An easy, simple inexpensive test for the specific detection of Pectobacterium carotovorum subsp . carotovorum based on sequence analysis of the pmrA gene Microbial genetics, genomics and proteomics. BMC Microbiology (2013) doi:10.1186/1471-2180-13-176
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More about the resource:
ClustalW2 web-based interface featured in this tutorial is provided by the European Bioinformatics Institute (EBI). The Clustal program was originally published by Thompson et al from Toby Gibson’s group in 1994, and was later updated by Larkin et al. ClustalW can be downloaded as source code or as binaries for various operating systems at www.clustal.org, provided by the Conway Institute at the University College of Dublin.
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